Effective microbiological control is a critical component to optimizing efficiencies in industrial cooling water systems and oftentimes the most difficult to achieve and maintain. Due to a wide range of reasons involving environmental discharge issues, worker safety concerns, storage, troublesome feed equipment, and impact on system metallurgy, the selection and/or application of micro biocides is becoming typical. A novel non-chemical means of maintaining total system microbiological control has been successfully developed and effectively applied to industrial cooling water systems. The process involves the unique and practical application of ultrasound and provides both planktonic and sessile biological control. This paper describes the treatment process as well as the advantages and benefits achieved by maintaining system cleanliness.